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High sensitivity CRP Human ELISA Kit

产品编号
BEK1239
产品规格
96T
产品应用
ELISA
产品价格
¥2800.00
产品介绍
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- hs-CRP antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- hs-CRP antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the hs-CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of hs-CRP can be calculated.
Kit components
1. One 96-well plate pre-coated with anti- Human hs-CRP antibody
2. Standard: 0.6ml (16000 ng/ml)
3. Standard diluent buffer: 2 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Biotin conjugated anti- Human hs-CRP antibody (RTU): 1.5 ml
6. HRP-conjugated reagent: 6ml
7. Stop solution: 6 ml
8. TMB substrate A:  6 ml
9. TMB substrate B:  6 ml
10. Plate sealer:   2
11. Hermetic bag:  1

技术参数

交叉反应: Human

产品来源: -

产品别名: -

产品成分: -

检测范围: 500 ng/ml-8000 ng/ml

检测样品: For quantitative detection of hs-CRP in Human serum, plasma, cell culture supernatant or tissue.

产品应用: ELISA

保存条件: 2-8℃6个月 -20℃一年

背景资料: C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation. The high-sensitivity C-reactive protein (hs-CRP) is a marker of inflammation, and in the detection of patients at increased risk for cardiovascular disease. hs-CRP is an independent predictor of future risk for cardiovascular events among healthy individuals, as well as among patients with acute coronary syndromes. In addition, because half of all cardiovascular events occur in persons with low to average levels of low-density lipoprotein cholesterol, hs-CRP may aid in identifying patients at high risk for a first cardiovascular event who might otherwise be missed by lipid screening alone. Thus, hs-CRP is a potential adjunct for global risk assessment in the primary prevention of cardiovascular disease.

参考资料: 1. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D (Dec 1991). "Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma". J Biol Chem 266 (32): 21833–8. 2. Tsai SP, Drayna D (Dec 1992). "The gene encoding human plasma carboxypeptidase B (CPB2) resides on chromosome 13". Genomics 14 (2): 549–50. 3. Sherif EM, Elbarbary NS, Abd Al Aziz MM, Mohamed SF. Plasma thrombin-activatable fibrinolysis inhibitor levels in children and adolescents with type 1 diabetes mellitus: possible relation to diabetic microvascular complications. Blood Coagul Fibrinolysis. 2014 Feb 6. 4. Wang S, Zhao Z, Cong Z, Suo G. Thrombin-activatable fibrinolysis inhibitor is activated in an instant blood-mediated inflammatory reaction after intraportal islet transplant. Exp Clin Transplant. 2014 Feb;12(1):62-6.

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