特异性斑马鱼抗体，可做WB, IHC, ICC等。
Catalog# BZA1033
Lot # Check on the product label
Size 50 μl
Host Rabbit
Reactivity
Human, mouse, ratSpecificity
Detect the endogenous levels of total GAPDH protein.Product Form LiquidImmunogen
Human GAPDH recombinant protein with full length.Recommend Application
Western Blot (1:5000-10,000)
Immunohistochemistry, IHC (1:100-500)
Immunocytochemistry, ICC (1:50-200)
Flow Cytometry (1:50-100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Aliquot and store at -20°C for 1 year.
Avoid repeated freeze and thaw cycles.
1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991.
2. Zheng L, Roeder RG, Luo Y (2003). "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2): 255–66.
3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18): 2606–2620.
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产品应用: WB, IHC, ICC
保存条件: Aliquot and store at -20°C for 1 year
背景资料: Background Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. Reference
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